用于生产流感病毒疫苗的动物细胞大规模培养技术凭借其高效稳定、易于放大、安全可控等优势，有望逐步取代传统的鸡胚培养法，成为生产流感疫苗的主流方法。深入认识流感病毒在疫苗生产细胞系中的病毒扩增与细胞应激特性，是指导生产细胞株的构建和病毒生产工艺的理性设计与优化的关键所在。此文概述了细胞基质流感病毒疫苗的生产流程，综述了流感病毒在生产细胞系中的扩增过程与生产细胞系感染流感病毒后的应激反应相关研究进展，并介绍了数学模型在流感病毒生产过程中的应用，旨在为细胞基质流感疫苗生产的理性优化提供参考。

The large-scale animal cell culture technology for production of influenza virus vaccines offers advantages such as high efficiency, stability, scalability, and safety, making it a promising alternative to traditional embryonated egg-based method. This approach is expected to become the prevailing method for manufacturing influenza virus vaccines. Gaining an in-depth understanding of the viral amplification and cellular stress responses of influenza viruses in vaccine production cell lines is pivotal for the development of production cell strains and the rational design and optimization of virus production processes. This review provides an overview of the production workflow for cell-based influenza vaccines, summarizes recent advancements in research on the viral amplification process and cellular stress responses following influenza virus infection in production cell lines, and highlights the application of mathematical modeling in influenza virus production. The aim is to offer insights and references to guide the rational optimization of cell-based influenza vaccine production processes.