随着微针技术的快速发展，微针逐渐成为新兴的疫苗递送方式，其具有微创、无痛、自给药、节省剂量、增强免疫应答等优势。免疫原性评价对疫苗的研发至关重要，目前尚无对微针疫苗免疫原性评价方法的系统性总结。此文概述了微针疫苗免疫应答的特点，系统梳理和讨论了用于评价微针疫苗免疫原性的各种方法及关键指标，并对新兴免疫原性评价方法在微针疫苗领域的应用进行了探讨和展望。

With the rapid development of microneedle technology, microneedle has gradually become a new vaccine delivery method, which has advantages such as minimally invasive, painless, self-administration, dose-saving, and enhanced immune response. The evaluation of immunogenicity is crucial for vaccine research and development. However, there is still no systematic summary of immunogenicity evaluation method of microneedle vaccines. This article summarizes the characteristics of immune responses to microneedle vaccines, systematically reviews and discusses various methods and key indicators for evaluating the immunogenicity of microneedle vaccines, as well as explores and prospects emerging immunogenicity evaluation methods in microneedle vaccines.