目的 验证细胞种属鉴别（多重PCR）法，并应用于多种不同种属来源的细胞鉴别。方法 使用细胞种属鉴别检测试剂盒（多重PCR法）提取细胞基因组DNA，用混合引物对靶基因进行扩增，采用多重PCR联合琼脂糖凝胶电泳技术，根据扩增产物的条带大小及数量进行物种鉴定及交叉污染检测，并考察该方法对5种不同来源细胞的检测限、专属性和耐用性。结果 细胞种属鉴别（多重PCR）法对Hep-2细胞（人）、犬肾细胞、Vero细胞（非洲绿猴）、L929细胞（小鼠）的检测限为500个细胞，对中国仓鼠卵巢细胞的检测限为5 000个细胞。对每种细胞设计不同来源的细胞进行交叉污染，主细胞及污染细胞均能检出，且能够检测出1‰水平的细胞污染。将5种细胞的基因组DNA提取液分别于﹣18 ℃保存1、3、7 d，扩增结果均一致。结论 该细胞种属鉴别（多重PCR）法灵敏度高、特异性强且快速便捷，有助于提高生物制品生产用细胞基质的质量控制水平。

Objective To validate the method of cell species identification (multiplex PCR) and apply it to the identification of cells from various species.Methods Cell genomic DNA was extracted by cell species identification detection kit (multiplex PCR), and the target gene was amplified by mixed primers. Multiplex PCR combined with agarose gel electrophoresis was used to identify species and detect cross-contamination according to the band size and number of amplified products, and the detection limit, specificity and durability to 5 different sources of cells were investigated.Results The detection limits of multiplex PCR method were 500 cells for Hep-2 cells (human), canine kidney cells, Vero cells (African green monkey) and L929 cells (murine), and 5 000 cells for Chinese hamster ovary cells. When each cell type was designed with cells from different sources for cross-contamination, both main cells and contaminant cells as low as 1‰ level were detected. When the genomic DNA extracts of 5 kinds of cells were stored at ﹣18 ℃ for 1, 3 and 7 d, the amplification results were consistent.Conclusion Multiplex PCR method has high sensitivity, strong specificity, rapidity and convenience, which is helpful to improve the quality control level of cell substrates for the production of biological products.