目的 评估3种Sabin株脊髓灰质炎灭活疫苗生产用片状载体的使用效果，为该疫苗生产工艺中载体的选择提供依据。方法 采用15 L篮式生物反应器体系，分别搭载A、B、C 3种片状载体进行Vero细胞培养并制备Sabin株脊髓灰质炎病毒液，考察并比较Vero细胞接种于片状载体后0~3 h的细胞贴附率、8 d培养周期的葡萄糖代谢特征、病毒收获液病毒滴度及D抗原含量等指标。结果 接种3 h后A、B、C 3种载体的细胞贴附率均达到90%以上。3组细胞均呈现典型S型葡萄糖消耗曲线，载体A和B组累计消耗量分别为（191.1±7.6） g和（192.9±5.3） g，差异无统计学意义（F=43.23，P=0.060），二者均高于C组的（153.1±4.4） g且差异有统计学意义（F=43.23， P<0.001）。载体B组D抗原含量为（777.0±12.5） D抗原单位（D-antigen unit，DU）/mL，病毒滴度为（8.2±0.1） lgCCID₅₀/mL，略高于载体A组〔（773.3±10.8） DU/mL，（8.1±0.2） lgCCID₅₀/mL〕，差异无统计学意义（F分别为209.00、27.30，P分别为0.946、0.708），而载体C组2项指标〔（598.3±14.2） DU/mL，（7.2±0.2） lgCCID₅₀/mL〕低于载体B组，差异有统计学意义 （F分别为209.00、27.30，P均小于0.001）。结论 载体A和载体B在Vero细胞代谢活性维持与病毒复制方面均较有优势。

Objective To evaluate the application effects of 3 types of sheet carriers for the production of Sabin strain inactivated poliovirus vaccine (sIPV) in order to provide basis for carrier selection in sIPV production process.Methods A 15 L basket bioreactor system was adopted to carry out Vero cell culture and prepare Sabin strain poliovirus liquid with 3 sheet carriers A, B, and C, respectively. The cell attachment rate of Vero cells inoculated on the sheet carriers within 0-3 h, the glucose metabolism characteristics during the 8-day culture cycle, the virus titer and D-antigen content of the virus harvest were investigated and compared.Results Cell attachment rates of carriers A, B, and C were all >90% at 3 h after inoculation. Cells in all 3 groups presented a typical S-shaped glucose consumption curve. The cumulative glucose consumptions of A and B were (191.1±7.6) g and (192.9±5.3) g, with no statistically significant difference (F=43.23, P=0.060), while both were statistically significantly higher than that of C at (153.1±4.4) g (F=43.23, P<0.001). D-antigen contents of A and B were (773.3±10.8) and (777.0±12.5) D-antigen unit (DU)/mL, and virus titers were (8.1±0.2), (8.2±0.1) lgCCID₅₀/mL,respectively, with no statistically significant difference (F=209.00, 27.30; P=0.946, 0.708). In contrast, the two indicators of group C at (598.3±14.2) DU/mL and (7.2±0.2) lgCCID₅₀/mL,were lower than those of group B with statistically significant differences (F=209.00,27.30; P<0.001).Conclusion Carriers A and B both exhibit clear advantages in maintaining the metabolic activity of Vero cells and promoting poliovirus replication.