作为分子生物学领域重要的核酸定量技术，数字PCR因其高灵敏度、高精确度以及高特异性等优势，近年来在病原体检测、基因编辑检测以及疾病诊断等领域得到了广泛应用。此文综述了数字PCR的基本概念、原理及其在生物检测领域的应用进展，以期为研究人员选择合适的检测方法提供参考。

As an important nucleic acid quantification technology in molecular biology, digital PCR has been widely used in the fields of pathogen detection, gene editing detection, and disease diagnosis in recent years due to its advantages of high sensitivity, accuracy, and specificity. This article reviews the basic concepts and principles of digital PCR, as well as the application progress of digital PCR in the field of biological detection, in order to provide a reference for researchers to choose appropriate detection methods.