目的 比较胰蛋白酶及胰蛋白酶类似物TrypLE对Vero细胞、2BS细胞、MDCK细胞的消化效果，以确定其在细胞培养及消化中的适用性。方法 分别用胰蛋白酶和TrypLE消化Vero细胞、2BS细胞、MDCK细胞，分析细胞消化时间、分散效果、消化残留情况、细胞计数、细胞活率，比较不同消化酶消化效果。将消化后细胞分别接种至细胞工厂和篮式生物反应器中进行培养，培养期间每天观察细胞工厂中细胞的形态以及汇合度，检测篮式生物反应器中细胞的贴附效果及葡萄糖消耗量。结果 使用胰蛋白酶和TrypLE消化Vero细胞及2BS细胞时，在消化时间、细胞计数及细胞活率方面差异均无统计学意义（t=﹣0.46~3.46,P=0.074~0.885），分散效果、消化残留情况相似。使用TrypLE消化MDCK细胞的时间明显长于使用胰蛋白酶，差异有统计学意义（t=13.28，P＜0.01），且消化效果较胰蛋白酶差。3种细胞消化后在细胞工厂再培养时，均形态规则，汇合度良好。在篮式生物反应器再培养时，3种细胞3 h内贴附率均在80%以上，且葡萄糖总代谢量差异无统计学意义（t=﹣1.05~1.01，P=0.405~0.783）。结论 TrypLE可以替代胰蛋白酶用于Vero细胞和2BS细胞的消化过程，而胰蛋白酶对MDCK细胞的消化效果更好。

Objective To compare the digestive effects of trypsin and trypsin-like enzyme TrypLE on Vero cells, 2BS cells, and MDCK cells, in order to determine their suitability for cell culture and digestion processes.Methods After trypsin solution and TrypLE were used to digest Vero cells, 2BS cells, and MDCK cells, key parameters such as digestion time, dispersion effect, residue after digestion, cell count, and cell viability were analyzed to compare the effects of different digestive enzymes. Digested cells were then inoculated into cell factories and basket bioreactors for cultivation. During the cultivation period, cell morphology and confluence in cell factory were observed daily, and the adhesion effect of cells and glucose consumption in basket bioreactor were measured.Results There were no statistically significant differences in digestion time, cell count, or cell viability of Vero cells and 2BS cells between trypsin and TrypLE digestions (t=﹣0.46-3.46, P=0.074-0.885), and dispersion effect and residue after digestion were similar. However, the digestion time for MDCK cells using TrypLE was statistically significantly longer than using trypsin (t=13.28, P＜0.01), and the digestion effect of TrypLE was inferior to that of trypsin. When these 3 types of cells were re-cultured in the cell factory after digestion, they all had regular shapes and good confluence. When re-cultured in the basket bioreactor, the adhesion rates of 3 types of cells within 3 h were all above 80%, and there was no statistically significant difference in the total glucose metabolism (t=﹣1.05-1.01，P=0.405-0.783).Conclusion TrypLE can replace trypsin for the digestion process of Vero cells and 2BS cells, while trypsin is more effective for digesting MDCK cells.