目的 在中国仓鼠卵巢（Chinese hamster ovary，CHO）细胞表达系统中真核表达截短型水痘-带状疱疹病毒（varicella zoster virus，VZV）糖蛋白E（glycoprotein E，gE），并评价其免疫原性。方法 将VZV-gE 1—546位氨基酸基因片段克隆至载体pCHO1.0，构建重组表达质粒 pCHO1.0-VZV-gE 1-546，瞬时转染至CHO细胞，扩增培养后收获上清。经离子交换层析获得高度纯化的重组截短gE，与复合佐剂（铝佐剂+CpG 1018佐剂）以一定比例混合，腹腔注射免疫小鼠2次，采血分离血清进行IgG抗体水平检测。末次免疫3周后安乐死小鼠，取脾脏淋巴细胞进行酶联免疫斑点试验检测。结果 基因测序结果表明，重组表达质粒pCHO1.0-VZV-gE 1-546构建正确；纯化后的重组截短gE的纯度可达95%以上。实验组小鼠免疫后血清中抗VZV的IgG抗体水平、IFN-γ和IL-2的斑点几何平均数均高于对照组小鼠，且复合佐剂组高于非佐剂组。结论 成功构建并表达了重组截短VZV gE，该蛋白在小鼠体内可诱导良好的免疫应答。

Objective To express the truncated glycoprotein E (gE) of varicella zoster virus (VZV) in eukaryotic cells using Chinese hamster ovary (CHO) cell expression system and evaluate its immunogenicity.Methods The VZV-gE 1-546 amino acid gene fragment was cloned into the vector pCHO1.0. The recombinant expression plasmid pCHO1.0-VZV-gE 1-546 was constructed and was transiently transfected into CHO cells, amplified and cultured, and the supernatant was harvested. Highly purified recombinant truncated gE, obtained through ion exchange chromatography, was mixed with a complex adjuvant (aluminum adjuvant + CpG 1018 adjuvant) in a certain proportion. Mice were immunized twice by intraperitoneal injection, and blood was collected to isolate serum for IgG antibody level detection. Three weeks after the last immunization, mice were euthanized and spleen lymphocytes were collected for enzyme-linked immunospot assay.Results Gene sequencing results showed that the recombinant expression plasmid pCHO1.0-VZV-gE 1-546 was constructed correctly, and the purity of the purified recombinant truncated gE reached over 95%. The IgG antibody level of anti-VZV and the geometric mean number of spots of IFN-γ and IL-2 cytokines in the serum of the experimental group mice after immunization were higher than those in the control group mice, and the complex adjuvant group was higher than the non-adjuvant group.Conclusion Recombinant truncated VZV gE is successfully constructed and expressed, which can induce a good immune response in mice.