肿瘤基因治疗虽然是非常有前景的方法，但是非细菌载体面临着转化效率低和递送脱靶的问题。使用肿瘤靶向的细菌作为载体是实体瘤基因治疗所倾向采用的策略之一。此文综述了基因修饰双歧杆菌在肿瘤靶向基因治疗中的新进展，包括基因修饰双歧杆菌介导的自杀基因治疗、抗血管增生治疗和肿瘤抑制基因治疗，也是首次对全球肿瘤基因治疗中的获批临床试验方案，特别是细菌载体方案趋势的综述。

Cancer gene therapy is a promising method to overcome cancer. But the nonbacterial vectors are in the face of the low efficient transduction and off-target delivery. The use of tumor-targeting bacteria as vectors is one of the preferentially strategies for the solid tumor gene therapy. This review summarizes the novel advances on the genetically modified Bifidobacterium (GMBif) for tumor-targeted gene therapy including GMBif-mediated suicide gene therapy, antiangiogenic gene therapy， and tumor suppressor gene therapy. The highlight was the first summary of the trend of approved clinical trial protocols in cancer gene therapy, especially the bacterial vector protocols worldwide.