目的 探索84消毒剂对乙型脑炎病毒的灭活效果并建立检测方法。方法 依据2002年国家卫生健康委员会发布的《消毒技术规范》，采用悬液定量法，在实验室代表性材料（玻璃、彩钢、聚氯乙烯、不锈钢）表面进行消毒剂-病毒相互作用，通过中和鉴定实验确定合适的中和剂，建立检测方法检测病毒活性，并对检测结果进行评价。结果 常温下，中和剂卵磷脂（10 g/L）、吐温 80（10 g/L）及其中和产物均导致宿主细胞（原代金黄地鼠肾细胞）死亡；中和剂硫代硫酸钠（1 g/L）及其中和产物对宿主细胞无毒性，对细胞生长无影响，对乙型脑炎病毒毒力无影响。故选择硫代硫酸钠（1 g/L）作为中和剂。用84消毒剂对乙型脑炎病毒进行病毒灭活实验，用硫代硫酸钠（1 g/L）中和后，用噬斑法对该混合物进行检测，未检测到乙型脑炎病毒。结论 有效氯含量为800 mg/L的84消毒剂与乙型脑炎病毒反应5 min后即可有效灭活病毒，灭活效果达到预期。

Objective To explore the inactivation effect of 84 disinfectant on Japanese encephalitis virus (JEV) and establish a detection method.Methods According to "Technical Specification for Disinfection" issued by National Health Commission in 2002, the suspension quantitative method was adopted to carry out the interaction between disinfectant and virus on the surfaces of representative laboratory materials (glass, colored steel, polyvinyl chloride, and stainless steel). Appropriate neutralizer was determined by neutralization identification tests. Detection method was established to detect virus activity, and the detection results were evaluated.Results At room temperature, neutralizers lecithin (10 g/L) and Tween 80 (10 g/L) , along with their neutralization byproducts, resulted in the death of host cells (primary golden hamster kidney cells). However, neutralizer sodium thiosulfate (1 mg/L) and its neutralization byproducts demonstrated no toxicity to the host cells, no impact on cellular proliferation, and no effect on the virulence of JEV. So sodium thiosulfate (1 g/L) was selected as neutralizer. Following the interaction between 84 disinfectant and the virus, and neutralization with sodium thiosulfate (1 g/L), plaque assay detection of the resultant mixture failed to identify any JEV particles.Conclusion The 84 disinfectant with available chlorine content of 800 mg/L can effectively inactivate JEV after reacting for 5 min, and the inactivation effect meets the expected goal.