目的 对柯萨奇病毒B组5型（coxsackievirus B5, CV-B5）的病毒蛋白1（viral protein 1, VP1）进行生物信息学分析，以期为后续免疫监测、表位疫苗设计和疾病研究提供科学依据。方法 应用ProtParam、SignalP-6.0、DeepTMHMM-1.0、NetPhos-3.1、NetNGlyc1.0、SOPMA、SWISS-MODEL、ABCpred、BCPred和IEDB在线网站预测分析VP1的物理化学性质、结构特征和线性B细胞抗原表位。结果 CV-B5 VP1是不稳定且呈碱性的亲水性蛋白，无信号肽结构，有1个跨膜结构域。预测该蛋白可能存在33个磷酸化位点和15个糖基化位点。VP1二级结构以无规则卷曲为主，存在多个潜在的优势Ｂ细胞抗原表位。结论 利用生物信息学工具对CV-B5 VP1进行预测分析，初步判断了VP1的生物学功能及线性抗原表位。

Objective To perform bioinformatic analysis of the viral protein 1 (VP1) of coxsackievirus B5 (CV-B5), in order to provide scientific basis for immune surveillance, epitope vaccine design and disease research.Methods Online websites including ProtParam, SignalP-6.0, DeepTMHMM-1.0, NetPhos-3.1, NetNGlyc1.0, SOPMA, SWISS-MODEL, ABCpred, BCPred, and IEDB were used to predict and analyze the physicochemical properties, structural characteristics and linear B-cell epitope of CV-B5 VP1.Results The CV-B5 VP1 appeared to be an unstable and alkaline hydrophilic protein with no signal peptide and one transmembrane domain. Thirty-three phosphorylation sites and 15 glycosylation sites were predicted in the protein. Random curling was the main secondary structure of VP1, and multiple potential dominant B-cell epitopes were identified.Conclusion Using bioinformatics tools to predict the CV-B5 VP1, the biological function and linear epitopes of VP1 are primarily identified.