目的　评估2021—2023年安徽省不同血清群的脑膜炎奈瑟菌(Neisseria meningitidis, Nm)对抗菌药物的敏感性，为临床治疗流行性脑脊髓膜炎（流脑）提供合理依据。方法　收集2021—2023年从安徽省的流脑病例、密切接触者和健康人群中分离培养的35株Nm，并采用E试验检测其对12种抗菌药物的敏感性。结果　35株Nm对青霉素和头孢噻肟的敏感率均为97.14%，对氨苄西林、头孢曲松、美罗培南、米诺环素、阿奇霉素、氯霉素及利福平的敏感率均为100.00%，对环丙沙星的敏感率仅为5.71%，对左氧氟沙星和复方新诺明的敏感率均为0.00%。其中，2022年从密切接触者中分离的1株B群Nm对青霉素不敏感，2023年从健康人群中分离的1株血清不可分群Nm对头孢噻肟不敏感。结论　安徽省2021—2023年分离的35株Nm对青霉素、氨苄西林、头孢噻肟、头孢曲松、美罗培南、米诺环素、阿奇霉素、氯霉素和利福平表现为普遍敏感，对环丙沙星、左氧氟沙星表现为部分耐药，对复方新诺明完全耐药。随着安徽省Nm耐药情况的日益复杂，应对Nm开展长期的耐药性监测以指导临床合理用药。

Objective　To evaluate the antimicrobial susceptibility of different serogroups of Neisseria meningitidis (Nm) in Anhui Province during 2021-2023， and to provide reasonable basis for clinical treatment of epidemic cerebrospinal meningitis.Methods　Thirty-five strains of Nm isolated and cultured from epidemic cerebrospinal meningitis cases, close contacts and healthy people in Anhui Province from 2021 to 2023 were collected, and their sensitivity to 12 kinds of antimicrobial agents was assessed by E-test.Results　The sensitivity rates of 35 Nm strains to 12 drugs were 97.14% for penicillin and cefotaxime, 100.00% for ampicillin, ceftriaxone, meropenem, minocycline, azithromycin, chloramphenicol and rifampicin, 5.71% for ciprofloxacin, and 0.00% for levofloxacin and trimethoprim-sulfamethoxazole. Among them, one group B Nm insensitive to penicillin was isolated from close contact in 2022, and one serogroup nongroupable Nm insensitive to cefotaxime was isolated from healthy person in 2023.Conclusions　The 35 Nm strains isolated in Anhui Province from 2021 to 2023 are generally sensitive to penicillin, ampicillin, cefotaxime, ceftriaxone, meropenem, minocycline, azithromycin, chloramphenicol and rifampicin, partially resistant to ciprofloxacin and levofloxacin, and totally resistant to trimethoprim-sulfamethoxazole. With the increasing complexity of Nm resistance in Anhui Province, long-term monitoring of Nm resistance should be carried out to guide clinical rational drug use.