目的 优化表达抗人IL-17A/F单克隆抗体（单抗）的中国仓鼠卵巢（Chinese hamster ovary，CHO）细胞的培养基，筛选获得高表达且质量可比抗体的国产培养基。方法 用1株表达抗IL-17A/F单抗的CHO细胞，首先筛选出较优的基础培养基和补料培养基组合，并对该组合进行进一步补料策略优化和2 L生物反应器筛选，再经反应器工艺确认比较抗体表达量和产品质量。结果 基础培养基BM-01、补料培养基FM-01和FM-02组合为重组CHO细胞表达抗IL-17A/F单抗最优的培养基组合；其中补料培养基FM-01补料策略为培养第3/6/8/10/12天（3%~7%），补料培养基FM-01∶FM-02的补加体积比例为10∶1。在2 L生物反应器中最终抗体表达量超过6.5 g/L，为原工艺的2倍以上，且产品质量一致性好。结论 使用基础培养基BM-01、补料培养基FM-01和FM-02组合，实现了抗IL-17A/F单抗在CHO细胞中的高表达且质量可比，为国产培养基用于规模化生产抗IL-17A/F单抗奠定了基础。

Objective To optimize the culture medium for Chinese hamster ovary (CHO) cells expressing anti-human IL-17A/F monoclonal antibody (anti-hIL-17A/F), and screen domestic culture media with high expression and comparable quality of antibody. Methods A CHO cell line expressing anti-hIL-17A/F was used to optimize the culture medium. The optimal basic medium and feed medium combination was selected, and the feed strategy optimization and 2 L bioreactor screening were carried out. Then the antibody expression and quality were measured in bioreactor final confirmation.Results The combination of basic medium BM-01, feed medium FM-01 and FM-02 was the optimal medium combination for the expression of anti-hIL-17A/F in recombinant CHO cells. Among them, the feed medium FM-01 was supplemented with a feed strategy of day3/6/8/10/12 (3%-7%), and the ratio of FM-01 to FM-02 was 10∶1. In 2 L bioreactor， the protein expression exceeded 6.5 g/L, more than twice of that of the original process, and the product quality was consistent.Conclusion High expression and comparable quality of anti-hIL-17A/F in CHO cells are achieved by using the combination of basic medium BM-01, feed medium FM-01 and FM-02, which lays a foundation for large-scale production of anti-hIL-17A/F using domestic culture media.