目的  验证和评价猪纤维蛋白原生产工艺中有机溶剂/去污剂（solvent/detergent，S/D）法联合干热法灭活病毒的有效性。方法  以辛德比斯病毒、乙型脑炎病毒和伪狂犬病毒为指示病毒，验证猪血浆S/D法灭活病毒的有效性；以脑心肌炎病毒和猪细小病毒为指示病毒，验证猪纤维蛋白原冻干制剂干热法灭活病毒的有效性。结果  S/D法中，辛德比斯病毒、乙型脑炎病毒和伪狂犬病毒清除滴度下降均大于4 lg；干热法中，脑心肌炎病毒和猪细小病毒清除滴度下降均大于4 lg。结论  S/D法和干热法联合能有效灭活上述指示病毒，可提高猪纤维蛋白原产品的安全性。

Objective  To verify and evaluate the efficacy of the solvent/detergent (S/D) method combined with dry-heating method to inactivate viruses in the preparation of porcine fibrinogen. Methods  Sindbis virus, Japanese encephalitis virus, and pseudorabies virus were used as indicator viruses for S/D virus inactivation. Encephalomyocarditis virus and porcine parvovirus were used as indicator viruses for dry-heating virus inactivation. Results  Reductions in titers of three indicator viruses by S/D method were ＞4 lg. Reductions in titers of two indicator viruses by dry-heating method were＞4 lg. Conclusion  S/D method and dry-heating method combined effectively inactivate the indicator viruses, thus can improve the safety of porcine fibrinogen.