Vaccination is recognized as the most effective measure to prevent influenza. In recent years, large-scale animal cell culture technology for viral vaccine production shows increasingly obvious advantages in safety, economy and timeliness, making this technology promisingly boost the industry for influenza vaccine manufacturing in future. How to use the key technology of large-scale animal cell culture to increase influenza virus yield is the key of enhancing these advantages. This review describes the influenza vaccine and its production methods, key technologies involved in large-scale animal cell culture-based influenza vaccine production, and factors affecting virus propagation efficiency in large-scale animal cell culture.