Objective  To preparate high-efficacy rabbit anti-severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) serum and to study the effect of different adjuvants in rabbit anti-SARS-CoV-2 serum preparation on the antibody titer. Methods  Nine New Zealand rabbits were randomly divided into groups a, b, and c, and were immunized by subcutaneous injections with SARS-CoV-2 mixed with Freund's adjuvant, aluminum hydroxide adjuvant, or monophosphoryl lipid A adjuvant, respectively. They were immunized 3 times on 0, 28, and 42 d. Blood samples were collected from the central ear artery before every immunization, and at 14 d after the first immunization. Blood was also collected from the carotid artery on 49 d after the first immunization to separate the serum for neutralizing antibody titer detection and neutralizing tests. Results  After the entire immunization process, all groups produced highly effective anti-SARS-CoV-2 serum. Group a (6 144.00) had a higher final neutralizing antibody titer than groups b (4 437.33) and c (3 754.67). The serum obtained from all 3 groups could effectively neutralize the KMS2 strain of SARS-CoV-2 in neutralizing tests without causing any cell damage. Conclusion  All 3 different adjuvants can be used to obtain highly effective anti-SARS-CoV-2 serum in New Zealand rabbits.