目的 分析重组HPV病毒样颗粒（virus-like particle，VLP）的二级结构。方法 取重组HPV16、18、52、58型VLP各1批，均调整蛋白浓度至250〜500 μg/ml，采用圆二色谱远紫外扫描，预测重组HPV VLP的二级结构。结果 重组HPV16 VLP二级结构的预测结果为α螺旋11.7%，反平行β折叠37.1%，平行β折叠4.5%，β转角19.2%，不规则卷曲30.3%；重组HPV18 VLP二级结构的预测结果为α螺旋11.9%，反平行β折叠36.9%，平行β折叠4.6%，β转角19.0%，不规则卷曲30.7%；重组HPV52 VLP二级结构的预测结果为α螺旋12.3%，反平行β折叠37.1%，平行β折叠4.7%，β转角19.0%，不规则卷曲30.2%；重组HPV58 VLP二级结构的预测结果为α螺旋11.7%，反平行β折叠35.3%，平行β折叠4.4%，β转角20.4%，不规则卷曲31.3%。结论 圆二色谱法预测了重组HPV16/18/52/58型VLP的二级结构，该技术可用于重组HPV VLP的结构确证研究。

Objective  To investigate the secondary structure of recombinant HPV virus-like particles (VLPs). Methods  One batch each of recombinant HPV16/18/52/58 VLPs was sampled and adjusted to 250-500 μg/ml protein concentration. The secondary structure of recombinant HPV VLPs were predicted by circular dichroism far UV scan. Results  The predicted secondary structures of recombinant HPV16/18/52/58 VLPs were as following：11.7% α-helical, 37.1% antiparallel β-sheet, 4.5% parallel β-sheet, 19.2% β-turn, 30.3% random coil for HPV16；11.9% α-helical, 36.9% antiparallel β-sheet, 4.6% parallel β-sheet, 19.0% β-turn, 30.7% random coil for HPV18; 12.3% α-helical, 37.1% antiparallel β-sheet, 4.7% parallel β-sheet, 19.0% β-turn, 30.2% random coil for HPV52, and 11.7% α-helical, 35.3% antiparallel β-sheet, 4.4% parallel β-sheet, 20.4% β-turn, 31.3% random coil for HPV58, respectively. Conclusions The secondary structure of recombinant HPV16/18/52/58 VLPs are predicted by circular dichroism. The circular dichroism technology could be utilized to investigate the structure of recombinant HPV VLPs.