目的  探讨不同性质的磷酸铝佐剂对9V型肺炎球菌多糖(Streptococcus pneumococcal 9V polysaccharide,9V-PS)结合物的免疫促进作用，及琥珀酸对免疫应答的影响。方法  采用3种磷酸铝佐剂与9V-PS结合物制备疫苗，另外在相同配方的疫苗中加入琥珀酸，同时将无佐剂的9V-PS结合物作为对照。用所制疫苗皮下免疫小鼠，收集不同针次免疫后血清，ELIS A测定免疫后小鼠血清中抗9V- PS IgG抗体浓度。结果  采用不同磷酸铝佐剂的3种疫苗之间比较，小鼠血清中抗9V- PS IgG抗体浓度差异无统计学意义(t值为0.38~0.86，P值均>0.05)。磷酸铝佐剂配伍的疫苗与无佐剂组相比，小鼠血清中抗9V-PS IgG抗体浓度均显著提高，差异有统计学意义(t值为3.75~4.35，P值均<0.05);佐剂A和B组加入琥珀酸与未加入比较，小鼠血清中抗9V- PS IgG抗体浓度差异无统计学意义(t值分别为1.39、0.45，P值均>0.05)。结论  磷酸铝佐剂可以显著提高9V-PS结合物的免疫应答。不同磷酸铝佐剂的免疫促进作用无明显差异。添加琥珀酸对免疫应答不产生明显影响。

Objective  To investigate the immune enhancement effect of different aluminum phosphate adjuvants on Streptococcus pneumococcal 9V polysaccharide (9V-PS) conjugate, and the influence of succinate buffer on immune response. Methods  Target antigen was formulated with three different aluminum phosphate adjuvants. Each formulation was further analyzed with succinate buffer. Target antigen without adjuvants was set as control group. Mice were immunized subcutaneously with prepared vaccines and serum samples were collected after each dose. Anti-9V-PS IgG antibody concentrations of the serum were analyzed by ELISA. Results  Anti-9V-PS IgG concentrations of mouse sera were not statistically significantly different among three vaccines formulated with different aluminum phosphate adjuvants (t values were 0. 38-0. 86, all P>0.05), but were significantly different (t values were 3. 75-4. 35, all P<0. 05) between vaccines formulated with and without aluminum phosphate adjuvants, higher in all adjuvant groups. Addition of succinate buffer did not affect anti-9V-PS antibody response in both adjuvants A and B groups (t values were 1. 39 and 0. 45, both P>0. 05). Conclusions  Aluminum phosphate adjuvants significantly improve immune response of 9V-PS conjugate, with no significant difference between different aluminum phosphate adjuvants. Addition of succinate buffer does not affect immune response.