目的 研究兔抗肠道病毒71型（enterovirus 71，EV71）血清制备中不同免疫剂量对抗体效价的影响。方法 将9只实验兔简单随机分为3组，每组3只。5、50和100 μg 3个剂量EV71灭活疫苗和弗氏佐剂均匀混合后，通过肌内注射途径分别免疫3组动物，于第0、7、14及28天各免疫1次，每7天采血，分离血清，检测抗EV71中和抗体效价。结果 100 μg剂量组兔血清抗体效价显著高于5和50 μg剂量组（第42天t值分别为–3.282和–2.486，P值均<0.05）。第42天时，兔血清抗体效价和免疫剂量（5~100 μg范围内）呈线性关系，回归方程y=478.229x–2 041.820。结论 在本实验选择的3个免疫剂量中，随着免疫剂量升高，产生的血清抗体效价也升高，且呈线性关系。

 Objective  To study the effect of different immune doses in rabbit anti-enterovirus 71 (EV71) serum preparation on the antibody titer. Methods  Three doses of 5, 50 and 100 μg were selected in this experiment. There were 3 groups of 3 rabbits in each group. After the mixture of EV71 inactivated vaccine and Freund's adjuvant, the rabbits in the three groups were immunized by intramuscular injection. The rabbits were immunized once at 0, 7, 14 and 28 d, respectively. Blood samples were collected every 7 d, serum was separated and neutralizing antibody titer was detected. Results  The antibody titer of 100 μg was significantly higher than that of 5 (t=−3.282) and 50 μg (t=−2.486) , on day 42 (P<0.05). There was a linear relationship between the titer of rabbit serum antibody and the immune dose (within the range of 5-100 μg). The linear model was established and the regression equation y=478.229x–2 041.820 was obtained. Conclusion  Among the 3 immune doses selected in this experiment, the titer of serum antibody increases with the increase of immune dose, and shows a linear relationship.