目的 比较15 L转瓶及40层细胞工厂工艺制备腮腺炎减毒活疫苗的病变情况、病毒滴度和原液各项质量指标。方法 分别采用15 L转瓶及40层细胞工厂培养原代鸡胚细胞1～3 d后感染S79株腮腺炎病毒，继续培养至5～7 d收获病毒液。两种培养方式收获的腮腺炎病毒单次收获液分别合并后获得疫苗原液，并进行相关检定。结果 转瓶收获的单次病毒液0和21 d检定的平均滴度分别为6.6、6.1 lg半数细胞培养感染量（50% cell culture infective dose，CCID₅₀）/ml。细胞工厂收获的单次病毒液0和21 d检定的平均滴度分别为6.8、6.2 lgCCID₅₀/ml。两种方式制备的腮腺炎减毒活疫苗原液各项质量指标均合格。结论 应用40层细胞工厂工艺制备腮腺炎减毒活疫苗的细胞病变情况及单次病毒收获液滴度均优于15 L转瓶培养工艺，此实验为40层细胞工厂制备腮腺炎减毒活疫苗的大规模生产及工艺改进奠定了基础。

Objective  To compare the cytopathic conditions,virus titers and quality indicators of live attenuated mumps vaccine prepared by using 15 L rolling bottle and 40-layer cell factory. Methods  Primary chicken embryo cells were cultured in 15 L rolling bottle and 40-layer cell factory, respectively. Cells were infected by the S79 mumps virus strain after 1-3 d of culture. And the viruses were harvested 5-7 d after further culture. The virus liquids harvested by two methods were concentrated to be the single harvests pooled virus liquids. The single harvests pooled virus liquids were subjected to control tests. Results  The mean titer of the virus cultured in rolling bottle was 6.6 lg 50% cell culture infective dose (CCID₅₀)/ml on 0 d, and 6.1 lgCCID₅₀/ml on 21 d. The mean titer of the virus cultured in cell factory was 6.8 lgCCID₅₀/ml at 0 d, and 6.2 lgCCID₅₀/ml on 21 d. All the quality indexes of live attenuated mumps vaccine prepared by two methods met the relevant requirements. Conclusions  The cytopathic conditions and virus titers of live attenuated mumps vaccine prepared by using cell factory are better than those in rolling bottle. The study lays foundation for large-scale production and technological improvement of live attenuated mumps vaccine prepared by using cell factory.