目的  研究水痘带状疱疹病毒（varicella-herpes zoster virus，VZV）Oka株的培养特性及对温度的敏感性。方法  对VZV Oka株感染的2BS细胞进行培养，当2BS细胞的致细胞病变效应（cytopathic effect，CPE）达到10%时，开始收集0、3、6、9、12、18、24、36 h的病毒悬液并检测病毒滴度，观察病毒生长曲线。从VZV培养的第1天起，每天检测病毒蛋白浓度，连续检测7 d，观察病毒蛋白浓度变化。将3、12、24、36 h收集的病毒悬液分别于4 ℃和25 ℃放置0、3、6、9、12、24 h，并检测病毒滴度。结果  在2BS细胞的CPE达到10%后0~12 h和12~24 h，病毒生长分别进入对数期和平台期，随后进入衰亡期。在VZV培养过程中，病毒蛋白在经历2 d的初始期后，于2~6 d进入对数期，随后进入平台期。不同生长时期的病毒置于4 ℃的滴度下降幅度和速度都小于置于25 ℃。结论  VZV Oka株具有一般病毒的培养特性，并受温度影响较大。

Objective  To study the culture characteristics and temperature sensitivity of varicella-zoster virus (VZV) Oka strain . Methods  2BS cells infected with VZV Oka strain were cultured. When the cytopathic effect (CPE) of 2BS cells reached 10%, the virus suspensions were harvested at 0, 3, 6, 9, 12, 18, 24, 36 h, and the virus titers were detected. The virus growth curve was observed. The virus protein concentrations were detected daily for 7 d since the first day of VZV culture, and the change of virus protein concentration was observed. The virus suspensions harvested at 3, 12, 24, 36 h were placed at 4 ℃ and 25℃ respectively for 0, 3, 6, 9, 12, 24 h, and the virus titers were detected. Results  The virus growth entered the log phase and plateau phase respectively at 0-12 h and12-24 h after the CPE of 2BS cells reached 10%, and then entered the decline phase. During VZV culture process, virus protein expression entered log phase at 2-6 d after 2 d initial phase, and then entered the plateau phase. The titer decline magnitude and speed of VZV in different growth periods placed at 4 °C were both lower than at 25 °C. Conclusion  VZV Oka strain has general virus culture characteristics, and is greatly affected by temperature.