Objective   To compare purification effect on human coagulation factor Ⅷ(FⅧ) between polyethylene glycol (PEG) precipitation and ion exchange chromatography. Methods  Three batches of FⅧ each were purified with PEG precipitation and ion exchange chromatography, respectively. Each quality index test was carried out according to Chinese Pharmacopoeia 2015 (volume Ⅲ). The recoveries and relative quality indexes of FⅧ purified by 2 purification processes were compared. Results  The recoveries of FⅧ purified by PEG precipitation and ion exchange chromatography were 29.90% and 68.25%, respectively. The specific activity and the tributyl phosphate residual amount of final FⅧ product were 3.8 IU/mg and 4.0μg/ml with PEG precipitation, and 61.8 IU/mg and 0.8 μg/ml with ion exchange chromatography. Conclusion  FⅧ purification with ion exchange chromatography can improve FⅧ quality effectively, and is more suitable for large scale production.