目的  利用猪血浆制备高纯度凝血酶。方法  使用DEAE-Sepharose FF阴离子交换层析分离猪血浆获得凝血酶原后，用C_aCl₂激活凝血酶原生成凝血酶。获得的凝血酶粗制品经2次SP-Sepharose FF阴离子交换层析获得高纯度凝血酶。结果  2次层析后的凝血酶的比活为2 546 IU/mg，是凝血酶粗制品的8倍，得率为54%。快速蛋白液相层析检测表明，2次层析后的凝血酶纯度达89%。结论  猪血浆通过1次DEAE-Sepharose FF和2次SP-Sepharose FF离子交换层析可得到高纯度凝血酶。

Objective  To prepare high purify thrombin from porcine plasma. Methods   Prothrombin was separated by DEAE-Sepharose FF anion-exchange chromatography from porcine plasma,and then was activated by CaCl₂ form thrombin. High purity thrombin was obtained by two-step SP-Sepharose FF anion-exchange chromatography from crude thrombin. Results  The specific activity of thrombin after two-step SP-Sepharose  FF canion-exchange chromatograph was 2 546 IU/mg, and 8 times of the crude thrombin activity. The recovery of thrombin was 54%. The result of fast protein liquid chromatography showed that the purity of thrombin after two-step SP-Sepharose FF canion-exchange  chromatograph was 89% . Conclusion  High purity thrombin can be obtained by DEAE-Sepharose FF and SP-Sepharose FF ion-exchange chromatography from porcine plasma.