目的  研究精子膜特异性蛋白Izumo基因mRNA在内蒙古白绒山羊睾丸和附睾组织中的表达。方法  采用逆转录-聚合酶链反应（reverse transcription-polymerase chain reaction，RT-PCR）法对内蒙古白绒山羊Izumo基因的部分片段进行重新克隆。然后重新设计一对引物，利用RNA印迹技术，以非放射性地高辛标记法对引物进行处理，制成地高辛标记DNA探针，与绒山羊睾丸和附睾头、体、尾部组织的总RNA进行杂交。 结果   RT-PCR扩增产物片段大小与预期的788 bp相符。RNA印迹试验中，标记的探针浓度为100 pg/µl，检测结果显示Izumo mRNA在内蒙古白绒山羊睾丸和附睾头、体、尾部组织均有表达。 结论   内蒙古白绒山羊睾丸和附睾组织均可表达Izumo mRNA，从而为进一步研究Izumo在人类精卵融合中的作用奠定了基础。

 Objective  To study expression of Izumo mRNA in testis and epididymis of Inner Mongolian white cashmere goats.  Methods   Part of the Izumo gene fragment was recloned by reverse transcription-polymerase chain reaction (RT-PCR). Then another pair of primers were designed. Digoxigenin-labeled DNA probes were prepared and hybridized with total RNA extracted from tissues of testis and head, body and tail of epididymis by Northern blotting.  Results  The size of the amplified fragment by RT-PCR was 788 bp. The concentration of the labeled DNA probe was 100 pg/μl and expression of Izumo mRNA was identified in tissues of testis and head, body and tail of epididymis.  Conclusion   The testis and epididymis of Inner Mongolian white cashmere goats can express Izumo mRNA, which lays foundation for further study of roles of Izumo in human sperm-egg fusion.