目的   观察疫苗生产中使用的消毒剂酸性苯酚和碱性苯酚对MRC-5细胞生长的影响。 方法   将酸性苯酚（0.37%）和碱性苯酚（0.74%）分别加入MRC-5细胞培养瓶中，观察细胞生长形态，绘制细胞生长曲线，测定细胞贴壁率。分别采用t检验和x²检验对结果进行比较。 结果  加入酸性和碱性苯酚后，第7天的细胞数分别为6.65×10⁵和7.12×10⁵，与对照组（6.93×10⁵）相比，差异无统计学意义（t=0.476，P=0.654；t=0.334，P=0.752）；第8小时的细胞贴壁率分别为88.3%和96.0%，与对照组（90.3%）相比，差异亦无统计学意义（x²=0.765，P=0.659；x²=0.376，P=0.585）。 结论  微量酸性和碱性苯酚对MRC-5细胞的生长没有显著影响。

 Objective   To investigate effect of acid phenol and alkaline phenol used in vaccine production as disinfectants on MRC-5 cell growth.  Methods   Acid phenol（0.37%） and alkaline phenol（0.74%）were added to MRC-5 cell culture, respectively. The morphology and growth curve of cells were observed, and cell attachment rate determined. The results were compared using t-test and x²-test.  Results  The number of MRC-5 cell added with acid phenol (6.65×10⁵) and alkaline phenol (7.12×10⁵) had no difference from that of control cells (6.93×10⁵) at 7 d (t=0.476，P=0.654；t=0.334，P=0.752). No difference of attachment rate was observed between the treated cells (88.3% and 96.0%) and control cells (90.3%) at 8 h (x²=0.765，P=0.659；x²=0.376，P=0.585).  Conclusion  Trace amount of acid and alkaline phenols has no significant effect on growth of MRC-5 cells.