目的   评价肠道病毒研究中常用的5种细胞对柯萨奇病毒A组16型（coxsackievirus A16，CA16）和肠道病毒71型（enterovirus 71，EV71）的敏感性，为分离和培养CA16和EV71提供实验室依据。方法  用分离的各15株CA16和EV71分别感染RD、Vero、KMB17、Hep2和L20B细胞，每天观察细胞病变情况，按Karber法计算病毒感染性滴度。采用不同的统计学方法（包括方差分析和卡方检验）比较5种细胞对CA16和EV71的敏感性差异。 结果   5种细胞的CA16和EV71病毒感染性滴度间的差异具有统计学意义（CA16：F＝18.481，P＝0.000；EV71：χ²＝63.106，P＝0.000）。在5种细胞中，RD细胞对2种病毒的敏感性最高，CA16和EV71的病毒感染性滴度分别可达7.8和8.2 lgCCID₅₀/ml，且均于接种细胞后48 h达增殖高峰，第4天进入增殖平台期；2种病毒在Hep2和L20B细胞中的病毒感染性滴度均较低，CA16分别为5.2和4.2 lgCCID₅₀/ml，EV71分别为4.9和4.0 lgCCID₅₀/ml，且2种病毒在接种后第6~7天才达增殖高峰，但仅L20B细胞中的2种病毒于接种后第7天进入增殖平台期；CA16在Vero和KMB17细胞中的病毒感染性滴度分别为6.9和7.3 lgCCID₅₀/ml，EV71分别为7.1和6.8 lgCCID50/ml，2种病毒均在接种Vero和KMB17细胞后第3天达到增殖高峰，第5天进入增殖平台期。 结论   RD细胞对CA16和EV71的敏感性最好，其次是Vero和KMB17细胞，而Hep2和L20B细胞并非是分离和培养这2种病毒的理想细胞。

 Objective   To evalute sensitivities of five kinds of cells to coxsackievirus A16 (CA16) and Enterovirus 71 (EV71) and provide laboratory evidences for isolation and culture of 2 viruses.  Methods   RD, Vero, KMB17, Hep2 and L20B cells were infected with 15 CA16 and 15 EV71 clinical isolates, respectively. The cytopathic effect of 5 cells were observed everyday. The infectious titers of CA16 and EV71 were calculated by the Karber method. The difference in sensitivity to CA16 and EV71 among 5 cells were compared with statistical methods, including one-way ANOVA and chi-square test.  Results  The infectious titers of CA16 and EV71 in 5 cells had significant difference (CA16：F＝18.481，P＝0.000；EV71：χ²＝63.106，P＝0.000). Of 5 cells, RD cell showed the highest sensitivity to 2 viruses, in which the infectious titers of CA16 and EV71 were 7.8 and 8.2 lgCCID50/ml respectively, and the proliferation of CA16 and EV71 reached peak at 48 h after inoculation and entered plateau phase 4 days after inoculation. The infectious titers of CA16 and EV71 in Hep2 and L20B were lower than thosse in other cells, and the infectious titers in Hep2 and L20B cells were 5.2 and 4.2 lgCCID₅₀/ml for CA16 and 4.9 and 4.0 lgCCID₅₀/ml for EV71, respectively. The proliferation of CA16 and EV71 reached peak 6-7 days after inoculation in L20B and Hep2 cells, but entered plateau phase 7 days after inoculation only in L20B cell. The infectious titers in Vero and KMB17 cell were 6.9 and 7.3 lgCCID₅₀/ml for CA16 and 7.1 and 6.8 lgCCID₅₀/ml for EV71, respectively. The proliferation of of CA16 and EV71 reached  peak 3 days after inoculation and entered plateau phase 5 days after inoculation.   Conclusion   Among five kinds Of 5 cells, RD cell has the best sensitivity to CA16 and EV71, and sensitivities of Vero and KMB17 cells are next, but Hep2 and L20B cells may not be ideal cells for isolation and culture of CA16 and EV71.