目的   研究ACYW135群脑膜炎球菌结合疫苗（groups A,C,Y and W135 meningococcal conjugate vaccine，MCV）在小鼠中的安全性及免疫原性。 方法   用4价MCV腹腔注射BALB/c小鼠，并于接种前及接种后7 d称量小鼠体重，观察小鼠的体重变化。分别于0和14 d用4价MCV皮下免疫BALB/c小鼠，并分别于第1剂接种后7和21 d采血，用间接ELISA测定血清特异性抗体水平。采用成组t检验对实验结果进行统计分析。 结果   在7 d观察期间，所有小鼠的体重均增加，4价MCV接种小鼠与对照小鼠体重增加间的差异无统计学意义（t=1.988，P>0.05）。100% 4价MCV接种小鼠的抗各群多糖抗体阳转，且抗体水平随接种次数的增加而提高。4价MCV接种小鼠与相应的单价疫苗原液接种小鼠的血清抗A群（t=2.107，P>0.05）、C群（t=1.343，P>0.05）、Y群（t=1.648，P>0.05）和W135群（t=0.556，P>0.05）抗体水平间的差异无统计学意义。 结论   4价MCV在小鼠中具有良好的安全性和免疫原性。

 Objective  To study the safety and immunogenicity of a groups A,C,Y and W135 meningococcal conjugate vaccine (MCV) in mice.   Methods   BALB/c mice were injected intraperitoneally with the tetravalent MCV and body weights of mice were measured before and 7 days after injection. BALB/c mice were injected subcutaneously with the tetravalent MCV on day 0 and 14, and blood was collected 7 and 21 days after first dose. Levels of serum antibodies against each polysaccharide were detected using indirect ELISA. The statistical analysis of the results were made by group t test.  Results   In the 7 days observation period, body weights of mice all increased, and weight gains had no significant difference between mice immunized with the tetravalent MCV and control mice (t=1.988, P>0.05). Serum conversion rates of antibodies against each polysaccharide were 100% in mice immunized with the tetravalent MCV, and levels of serum antibodies against each polysaccharide increased with the increase of injection times. Levels of serum antibodies against group A (t=2.107, P>0.05), group C (t=1.343, P>0.05), group Y (t=1.648, P>0.05) and group W135 (t=0.556, P>0.05) had no significant difference between mice immunized with the tetravalent MCV and relevant monovalent vaccine bulk.  Conclusion   The tetravalent MCV has good safety and immunogenicity in mice．