目的   制备高滴度呼肠孤病毒3型（reovirus 3，Reo-3）病毒液，并检测Reo-3滴度。 方法   以幼仓鼠肾细胞（BHK-21）为病毒培养基质和滴度测定细胞。将Reo-3按10⁰至10^-5感染复数（MOI）接种BHK-21，再分别用细胞病变法和蚀斑形成法检测病毒，用Karber法计算病毒滴度。 结果   BHK-21感染Reo-3后能产生明显病变。以10^-4 MOI的Reo-3接种后48 h，收获液中病毒滴度最高，细胞病变法检测为9.625 lgTCID50/ml，蚀斑形成法检测为8.671 lgPFU/ml。 结论   制备了高滴度Reo-3，为开展该病毒去除灭活研究奠定了基础。

 Objective   To prepare high-titer reovirus 3 (Reo-3) and determine its titer.  Methods  Baby hamster kidney cell (BHK-21) was used for virus cultivation and titration. The cells were infected with 100 to 10-5 multiplicity of infection (MOI) of Reo-3. The virus was detected by cytopathic effect (CPE) method and plaque forming assay, respectively. Virus titers were calculated with Karber formula.  Results  The CPE on infected BHK-21 cells was easily observed. The highest virus titers, 9.625 lgTCID50/ml tested by CPE method and 8.671 lgPFU/ml tested by plaque forming assay, were obtained when BHK-21 cells were inoculated by 10-4 MOI of Reo-3 48 h after infection.  Conclusion   High-titer Reo-3 can be prepared, which lays the foundation for study on virus inactivation and removal.