人呼吸道合胞病毒（resipiratory syncytial virus，RSV）是世界范围内儿童下呼吸道感染的主要病原，但其疫苗的开发面临许多困难和挑战。随着反向遗传学及相关技术和工具的应用，RSV疫苗，尤其是减毒活疫苗的研制已经取得很大进展。此文就RSV减毒活疫苗研发的主要突破和研究进展进行综述。

 Human respiratory syncytial virus (RSV) is the main pathogen of lower respiratory tract infection of children around the world, but the development of the vaccine is facing many difficulties and challenges. With the application of reverse genetics and related technology, the development of RSV vaccine has made great progress, especially in live-attenuated vaccines development. In this paper, the major breakthrough and research progress of live-attenuated RSV vaccine development are reviewed.