Abstract：Objective To purify Sabin strain poliovirus (sPV) using new multimodal chromatography medium Capto Core 400 and traditional medium Sepharose 6FF to remove residual host cell protein (HCP) and DNA, comparing purification results and process parameters. Methods Types Ⅰ, Ⅱ, Ⅲ sPV concentrates were purified using Capto Core 400 and Sepharose 6FF media, respectively. D antigen contents, HCP and DNA residues in purified sPV liquids were detected. D antigen recovery rates, as well as HCP and DNA removal rates were caculated. Results D antigen recovery rates of types Ⅰ, Ⅱ, Ⅲ sPV purified by Capto Core 400 and Sepharose 6FF had no statistically significant difference (t values were 1.09, 1.08, and 1.02, respectively, all P values>0.05). But HCP residue (t values were 3.15, 3.23 and 3.54, respectively) and cell DNA residue removal rates (t values were 3.41, 3.25 and 3.62, respectively) of the former were all higher than the latter, all with statistically significant differences (all P values<0.05). The specific values for Capto Core 400 and Sepharose 6FF process parameter ratios were: medium volume 1/20, purification time 1/25, application volume of buffer 1/12, process linear velocity 10, loading quantity of sample per litre medium 20, maximum withsatand pressure 2. Conclusion Comparing with Sepharose 6FF, Capto Core 400 removes residual Vero cell HCP and DNA more effectively, with more highly optimized process parameters, resulting in increased sPV purification process efficiency and lowered time and cost.
2020, Vol. 43 
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