目的  研制23价肺炎链球菌荚膜多糖疫苗。方法  大罐培养1、2、3、4、5、6B、7F、8、9N、9V、10A、11A、12F、14、15B、17F、18C、19A、19F、20、22F、23F、和33F型肺炎链球菌并进行各型荚膜多糖的纯化，制备23价肺炎链球菌荚膜多糖疫苗。 结果  经检定各型精制多糖主要质量指标均符合《欧洲药典》（2005
年版）要求。 结论  已建立起成熟的细菌培养和多糖纯化工艺。

Objective  To prepare 23-valent pneumococcal capsular polysaccharide vaccine.   Methods   Pneumococci types 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F were cultivated in the large fermenter and various types of capsular polysaccharides were purified to prepare 23-valent pneumococcal capsular polysaccharide vaccine.   Results  The main quality indices of the various types of purified polysaccharides conformed to the requirements of European Pharmacopoeia (2005).  Conclusion  The mature processes of bacterial cultivation and polysaccharide purification are established.