目的  探讨核心蛋白聚糖（decorin, DCN）联合白细胞介素24（interleukin-24, IL-24）对人外周血单个核细胞（human peripheral blood mononuclear cell, PBMC）增殖及分泌干扰素γ（Interferon-γ, IFN-γ）的影响。方法  构建重组质粒pcDNA3.1（+）- DCN和 pcDNA3.1（+）- IL-24，并利用脂质体将两种质粒转染PBMC。根据转染质粒的不同将PBMC分为空白对照组、脂质体组、空质粒组、DCN组、IL-24组、DCN与IL-24联合组。采用四甲基偶氮唑蓝比色法检测PBMC的增殖，ELISA测定细胞培养上清中IFN-γ的表达，流式细胞技术检测PBMC表面程序性死亡分子1（PD-1）的表达。采用LSD-t检验进行统计学分析。 结果  重组质粒pcDNA3.1（+）- IL-24构建成功。与其他组相比，DCN与IL-24联合能显著提高PBMC增殖率和IFN-γ分泌量，同时也能上调PD-1的表达水平。 结论  DCN与IL-24双基因联合在体外能促进PBMC的增殖，并能增强PBMC的功能。

Objective  To investigate the co-effect of decorin（DCN）and interleukin-24（IL-24）on proliferation and Interferon-γ (IFN-γ) secretion of human peripheral blood mononuclear cells（PBMCs）. Methods  Recombinant plasmid pcDNA3.1（+）- DCN and pcDNA3.1（+）- IL-24 were constructed and transfected into PBMCs by liposome. After transfected with different plasmids, the PBMCs were divided into 6 groups：blank control group, LipofectamineTM group, empty vector group, DCN group, IL-24 group, DCN and IL-24 group. PBMC proliferation was determined by methyl thiazolyl tetrazolium assay. IFN-γ expression in the supernatant was detected by ELISA. Flow cytometry was used to determine the surface expression of programmed death-l (PD-1) on PBMCs. Statistical analysis was made using LSD-t test. Results  The recombinant plasmid pcDNA3.1（+）-IL-24 was constructed successfully. PBMC proliferation and IFN-γ secretion were significantly higher in DCN and IL-24 group, and the expression of PD-1 was also upregulated obviously.  Conclusion  The combination of DCN and IL-24 could promote PBMC proliferation and strengthen the function of PBMCs in vitro.