目的  对A、C、Y和W135 4个不同血清群脑膜炎球菌菌株的16S rRNA、PorA和PorB基因进行序列分析，从分子水平对其做出鉴定。方法  提取脑膜炎球菌基因组DNA为
模板，设计特异性引物进行16S rRNA、PorA和PorB基因的PCR扩增并测序。结果  A、C、Y和W135群4个菌株的16S rRNA基因序列与GenBank中奈瑟菌属的脑膜炎球菌的同源性最高。4个菌株的PorA基因分型分别为P1.5-2,10; P1.7-1,1; P1.5-1,2-2; P1.5,2。PorB基因分别与porB3-26、porB2-40、porB3-100和porB3-25同源。结论  从基因水平分析表明4个菌株属于脑膜炎球菌，并在PorA和PorB基因分型上有差异。

 Objective  To analyze the sequences of 16S rRNA, PorA and PorB genes in 4 different Neisseria meningitidis strains of group A, C, Y and W135, and to identify the 4 strains at the molecular level.  Methods  The genomic DNAs were extracted from the 4 strains and used as templates. The 16S rRNA, PorA and PorB genes were amplified by PCR employing specific primers and sequenced. Results  The 3 genes could be amplified from all of the 4 strains. The 16S rRNA genes had the best homology with that of Neisseria meningitides. The PorA genotypes of the 4 strains were P1.5-2,10; P1.7-1,1; P1.5-1,2-2; P1:5,2. The PorB genes showed homology with alleles porB3-26, porB2-40, porB3-100 and porB3-25，respectively. Conclusions  The 4 strains are proved to be Neisseria meningitidis by gene sequencing, and show difference of the PorA and PorB genotypes.