Objective  To establish a production process for purifying virus in quadrivalent influenza vaccine using domestic fillers. Methods  Imported filler Sepharose 4FF and domestic filler Seplife 4FF were used to purify the multivalent influenza vaccine, respectively. The purified virus liquid underwent comprehensive evaluation of the separation and purification effect through single radial immunodiffusion assay, ELISA and electron microscope observation. Results  The recovery rate of hemagglutinin by Seplife 4FF was higher than that of Sepharose 4FF and removal rates of ovalbumin were similar at 50 cm/h flow rate. The 95% confidence intervals of removal rate for ovalbumin and recovery rate for hemagglutinin of Seplife 4FF for the four types of viruses were 91.5%-94.6% and 92.4%-100.8%, respectiely, with consistent results of electron microscope observation for both fillers. Conclusion  A method using domestical filler to efficiently purify influenza virus has been successfully established.