Objective  To construct and identify the recombinant BCG vaccine with nucleocapsid(N) protein gene of severe acute respiratory syndrome coronavirus 2（SARS-CoV-2）. Methods  SARS-CoV-2 N protein gene was obtained from plasmid pUC57-N(Kan⁺) by PCR. The N gene was connected to the shuttle expression plasmid pMV261 of Escherichia coli and BCG vaccine, and identified by enzyme digestion, PCR and sequencing. The recombinant plasmid was transformed into the competent state of BCG, and the expression of the target antigen in the recombinant BCG was identified by Western blot. Results  N gene with 1 260 bp was obtained by PCR amplification, and the recombinant plasmid pMV261-N had complete sequense,and the fragment inserted was consistent with N gene full size and sequence of America National Center for Biotechnology Information. The recombinant plasmid could express the target protein in BCG, and its relative molecular weight was about 46 000. Western blot showed that the protein could be recognized by anti-N protein monoclonal antibody. Conclusion  The recombinant BCG can express the fusion protein stably, which lays the foundation for the development of recombinant SARS-CoV-2 N protein BCG vaccine.